Trunk diseases significantly limit the productivity and longevity of vineyards in most of the winegrowing regions all over the world. Pathogens - a set of taxonomically- unrelated Ascomycete fungi - associated with grapevine trunk diseases are able to infect healthy vines mainly by pruning wounds and these wounds can remain susceptible for several months. It is important to highlight that there are no curative methods to control GTDs; the only way is to prevent or limit the infection of wood diseases using different cultural practices. The prevention of wound infection applying biocontrol agents is one of an alternative technique to control trunk diseases. Species of the genus Trichoderma (an ascomycete fungus, originally present in the soil) have been investigated several times as a potential biocontrol agent by spatial and nutritive competition.