Grapevine trunk diseases (GTDs), caused by a complex of pathogenic fungi, have increased especially following the prohibition of chemical products as sodium arsenite (for Esca) first and the Benzimidazoles Benomyl and carbendazim, later. Their eradication is not simple: currently, no efficient treatment exists. For this reason and in order to avoid GTDs spread, prophylactic methods should be used and implemented both in nursery and vineyard, since its planting.